Medium-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADM), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 26-421aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: KANRQREPGL GFSFEFTEQQ KEFQATARKF AREEIIPVAA EYDKTGEYPV PLIRRAWELG LMNTHIPENC GGLGLGTFDA CLISEELAYG CTGVQTAIEG NSLGQMPIII AGNDQQKKKY LGRMTEEPLM CAYCVTEPGA GSDVAGIKTK AEKKGDEYII NGQKMWITNG GKANWYFLLA RSDPDPKAPA NKAFTGFIVE ADTPGIQIGR KELNMGQRCS DTRGIVFEDV KVPKENVLIG DGAGFKVAMG AFDKTRPVVA AGAVGLAQRA LDEATKYALE RKTFGKLLVE HQAISFMLAE MAMKVELARM SYQRAAWEVD SGRRNTYYAS IAKAFAGDIA NQLATDAVQI LGGNGFNTEY PVEKLMRDAK IYQIYEGTSQ IQRLIVAREH IDKYKN. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: This enzyme is specific for acyl chain lengths of 4 to 16. Reference: "Nucleotide sequence of medium-chain acyl-CoA dehydrogenase mRNA and its expression in enzyme-deficient human tissue."Kelly D.P., Kim J.-J.P., Billadello J.J., Hainline B.E., Chu T.W., Strauss A.W.Proc. Natl. Acad. Sci. U.S.A. 84:4068-4072(1987). Function: Acyl-CoA dehydrogenase specific for acyl chain lengths of 4 to 16 that catalyzes the initial step of fatty acid beta-oxidation. Utilizes the electron transfer flavoprotein (ETF) as an electron acceptor to transfer electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).