Matrix protein (M), recombinant human metapneumovirus

Organism: Human metapneumovirus (strain CAN97-83) (HMPV). Source: Mammalian cell. Expression Region: 1-254aa. Protein Length: Full Length. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: MESYLVDTYQ GIPYTAAVQV DLVEKDLLPA SLTIWFPLFQ ANTPPAVLLD QLKTLTITTL YAASQSGPIL KVNASAQGAA MSVLPKKFEV NATVALDEYS KLEFDKLTVC EVKTVYLTTM KPYGMVSKFV SSAKPVGKKT HDLIALCDFM DLEKNTPVTI PAFIKSVSIK ESESATVEAA ISSEADQALT QAKIAPYAGL IMIMTMNNPK GIFKKLGAGT QVIVELGAYV QAESISKICK TWSHQGTRYV LKSR. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Has a crucial role in virus assembly and budding. The matrix interacts with the RNP complex and this association serves two functions: facilitate virion assembly and inhibit the viral transcriptase activity. Early in infection, M is localized to the nucleus and may inhibit host cell transcription. Later on, M can associate with lipid rafts supposely by interacting with the cytoskeleton and with the cytoplasmic tail of glycoprotein G. The binding of M to host membrane is stabilized by the surface expression of the viral glycoproteins. These interactions may allow virus formation by mediating association of the nucleocapsid with the nascent envelope. Reference: n/a. Function: nan