Matrix protein (M), Rabies virus, recombinant

Organism: Rabies virus (strain PM1503/AVO1) (RABV). Source: E.coli. Expression Region: 1-202aa. Protein Length: Full Length. Tag Info: N-terminal GST-tagged. Target Protein Sequence: MNVLRKIVKK CRDEDTQKPS PVSAPPDDDD LWLPPPEYVP LKELTSKKNM RNFCVNGDVK ACSPNGYSFR ILRHILRSFN EIYSGNHRMI GLVKVVVGLA LSGAPVPEGM NWVYKLRRTL IFQWADSRGP LEGEELEYSQ EITWDDDTEF VGLQIRVSAR QCHIQGRIWC INTNSRACQL WSDMSLQTQR SEEDKDSSLL LE. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Plays a major role in assembly, budding and uncoating of virion after membrane fusion. Completely covers the ribonucleoprotein coil and keep it in condensed bullet-shaped form. Inhibits viral transcription and stimulates replication. Plays a major role in early induction of TRAIL-mediated apoptosis in infected neurons. Reference: "Sequence of the 3386 3' nucleotides of the genome of the AVO1 strain rabies virus: structural similarities in the protein regions involved in transcription." Poch O., Tordo N., Keith G. Biochimie 70:1019-1029(1988). Function: nan