MAP1LC3B/LC3B (human recombinant)

Microtubule-associated protein 1 light chain 3beta (MAP1LC3B), also known as LC3B, is an ortholog of yeast Atg8 that is essential to autophagosome biogenesis. LC3B is irreversibly cleaved by the cysteine protease ATG4B (Cay-25380) to its active form LC3B-I, which contains an exposed C-terminal glycine residue needed for autophagosome formation. LC3B-I is then conjugated to phosphatidylethanolamine in canonical autophagy by the ATG12-ATG5-ATG16 complex to form LC3B-II, which localizes to the autophagosomal membrane.{48159, 47096,47097,47098} Knockdown of LC3B increases the number of ATG5- and ATG16-positive puncta in HeLa cells, indicating a defect in the maturation of autophagosomes. LC3B expression is upregulated in various cancers and has disease-specific implications on progression and prognosis. In metastatic and castration-resistant human prostate cancer tumor samples, LC3B expression is upregulated and is positively correlated with reduced disease-specific mortality. Expression of LC3B is also increased in oral squamous cell carcinomas and is associated with aggressive pathological features and poor prognosis.Synonyms: Autophagy-related Protein LC3B, Autophagy-related Ubiquitin-like Modifier LC3B, MAP1 Light Chain 3-like Protein 2, Microtubule-associated Protein 1 Light Chain 3beta. Purity: >95% estimated by SDS-PAGE. Source: Recombinant N-terminal His-tagged MAP1LC3B/LC3B purified from E. coli. Amino Acids: 2-120. MW: 16.22 kDa. Formulation: (Request formulation change), 50 mM HEPES, pH 8.0, with 150 mM sodium chloride, and 10% glycerol. UniProt Accession #: Q9GZQ8.