Mannan-binding lectin serine protease 2 (MASP2), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 16-686aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: TPLGPKWPEP VFGRLASPGF PGEYANDQER RWTLTAPPGY RLRLYFTHFD LELSHLCEYD FVKLSSGAKV LATLCGQEST DTERAPGKDT FYSLGSSLDI TFRSDYSNEK PFTGFEAFYA AEDIDECQVA PGEAPTCDHH CHNHLGGFYC SCRAGYVLHR NKRTCSALCS GQVFTQRSGE LSSPEYPRPY PKLSSCTYSI SLEEGFSVIL DFVESFDVET HPETLCPYDF LKIQTDREEH GPFCGKTLPH RIETKSNTVT ITFVTDESGD HTGWKIHYTS TAQPCPYPMA PPNGHVSPVQ AKYILKDSFS IFCETGYELL QGHLPLKSFT AVCQKDGSWD RPMPACSIVD CGPPDDLPSG RVEYITGPGV TTYKAVIQYS CEETFYTMKV NDGKYVCEAD GFWTSSKGEK SLPVCEPVCG LSARTTGGRI YGGQKAKPGD FPWQVLILGG TTAAGALLYD NWVLTAAHAV YEQKHDASAL DIRMGTLKRL SPHYTQAWSE AVFIHEGYTH DAGFDNDIAL IKLNNKVVIN SNITPICLPR KEAESFMRTD DIGTASGWGL TQRGFLARNL MYVDIPIVDH QKCTAAYEKP PYPRGSVTAN MLCAGLESGG KDSCRGDSGG ALVFLDSETE RWFVGGIVSW GSMNCGEAGQ YGVYTKVINY IPWIENIISD F. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase. Reference: "Proteolytic activities of two types of mannose-binding lectin-associated serine protease." Matsushita M., Thiel S., Jensenius J.C., Terai I., Fujita T. J. Immunol. 165:2637-2642(2000). Function: nan