Malto-oligosyltrehalose trehalohydrolase 13A, Nostoc punctiforme

Malto-oligosyltrehalose trehalohydrolase 13A (NpTrz13A), assigned the E.C. number 3.2.1.141, is a derivative of Nostoc punctiforme. It is an enzyme that participates in the hydrolysis of 1,4-alpha-D-glucosidic linkage in 4-alpha-D-(1,4-alpha-D-glucanosyl)(n) trehalose to yield trehalose and 1,4-alpha-D-glucan. The recombinant NpTrz13A, purified from Escherichia coli, is a modular Glycoside Hydrolase family 13 (GH13) enzyme, with an N-terminal family 48 Carbohydrate Binding Module (CBM48) (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,5 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Glycoside Hydrolase. Activity: Trehalases. Family: GH13. Ec Number: 3.2.1.141. Organism: Nostoc punctiforme. Architecture: CBM48-GH13. Optimal pH: 7. Optimal Temperature: 25°C. Substrates: 1,4-alpha-glucans, maltohexaose