Major structural protein p17 (Ba71V-107), African swine fever virus, recombinant

Organism: African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV). Source: in vitro E.coli expression system. Expression Region: 1-117aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MDTETSPLLS HNLSTREGIK QSTQGLLAHT IARYPGTTAI LLGILILLVI ILIIVAIVYY NRSVDCKSSM PKPPPSYYVQ QPEPHHHFPV FFRKRKNSTS LQSHIPSDEQ LAELAHS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Essential for the correct processing of both structural polyproteins and for the maturation of viral precursor membranes at the viral factories. Reference: "African swine fever virus protein p17 is essential for the progression of viral membrane precursors toward icosahedral intermediates." Suarez C., Gutierrez-Berzal J., Andres G., Salas M.L., Rodriguez J.M. J. Virol. 84:7484-7499(2010). Function: nan