Major centromere autoantigen B (CENPB), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-599aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: GPKRRQLTFR EKSRIIQEVE ENPDLRKGEI ARRFNIPPST LSTILKNKRA ILASERKYGV ASTCRKTNKL SPYDKLEGLL IAWFQQIRAA GLPVKGIILK EKALRIAEEL GMDDFTASNG WLDRFRRRHG VVSCSGVARA RARNAAPRTP AAPASPAAVP SEGSGGSTTG WRAREEQPPS VAEGYASQDV FSATETSLWY DFLPDQAAGL CGGDGRPRQA TQRLSVLLCA NADGSEKLPP LVAGKSAKPR AGQAGLPCDY TANSKGGVTT QALAKYLKAL DTRMAAESRR VLLLAGRLAA QSLDTSGLRH VQLAFFPPGT VHPLERGVVQ QVKGHYRQAM LLKAMAALEG QDPSGLQLGL TEALHFVAAA WQAVEPSDIA ACFREAGFGG GPNATITTSL KSEGEEEEEE EEEEEEEEGE GEEEEEEGEE EEEEGGEGEE LGEEEEVEEE GDVDSDEEEE EDEESSSEGL EAEDWAQGVV EAGGSFGAYG AQEEAQCPTL HFLEGGEDSD SDSEEEDDEE EDDEDEDDDD DEEDGDEVPV PSFGEAMAYF AMVKRYLTSF PIDDRVQSHI LHLEHDLVHV TRKNHARQAG VRGLGHQS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes (Probable). Reference: "The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. Rogers J. Nature 414:865-871(2001). Function: nan