Lys-gingipain 381 (kgp), partial, Porphyromonas gingivalis, recombinant

Organism: Porphyromonas gingivalis. Source: E.coli. Expression Region: 244-594aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MLVVAGAKFK EALKPWLTWK AQKGFYLDVH YTDEAEVGTT NASIKAFIHK KYNDGLAASA APVFLALVGD TDVISGEKGK KTKKVTDLYY SAVDGDYFPE MYTFRMSASS PEELTNIIDK VLMYEKATMP DKSYLEKALL IAGADSYWNP KIGQQTIKYA VQYYYNQDHG YTDVYSYPKA PYTGCYSHLN TGVGFANYTA HGSETSWADP SLTATQVKAL TNKDKYFLAI GNCCVTAQFD YPQPCFGEVM TRVKEKGAYA YIGSSPNSYW GEDYYWSVGA NAVFGVQPTF EGTSMGSYDA TFLEDSYNTV NSIMWAGNLA ATHAGNIGNI THIGAHYYWE AYHVLGDGSV M. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Cysteine proteinase with a strong preference for substrates with Lys in the P1 position. Hydrolyzes bovine hemoglobin, bovine serum albumin, casein, human placental type I collagen and human IgA and IgG. Disrupts the functions of polymorphonuclear leukocytes. May act as a virulence factor in the development of peridontal disease. Involved in the coaggregation of P.gingivalis with other oral bacteria. Reference: "Distribution of Porphyromonas gingivalis biotypes defined by alleles of the kgp (Lys-gingipain) gene." Nadkarni M.A., Nguyen K.A., Chapple C.C., DeCarlo A.A., Jacques N.A., Hunter N. J. Clin. Microbiol. 42:3873-3876(2004). Function: nan