LOXL2, Recombinant, Human (Lysyl Oxidase-like Protein 2, Lysyl Oxidase Related Protein 2, LOR2, Lysy

Lysyl Oxidase Homolog 2 (lysyl oxidase-like protein 2, LOXL2) is a member of lysyl oxidase-like (LOXL) gene family which includes LOXL1 through LOXL4. These enzymes are secreted copper-binding amine oxidases that oxidize primary amine substrates to aldehydes. The N-terminal region of LOXL2 contains four scavenger receptor cysteine- rich (SRCR) domains, and the C-terminal region is a catalytic domain similar to other lysyl oxidases. The catalytic domain contains conserved residues required for copper binding and formation of a lysyl tyrosylquinone co-factor. Although some of the LOXL enzymes are known to cross-link collagen and elastin substrates, such a function has yet to be characterized for LOXL2. It has been shown that LOXL2 promotes cell migration and tumor cell invasiveness. Elevated expression of LOXL2 is also associated with cancer progression in various tumors and carcinoma cell lines, which makes it a potential marker for prognosis of cancer. LOXL2 is expressed in many tissues, with elevated levels in reproductive tissues such as placenta, uterus, and prostate. Source: Murine myeloma cell line, NS0-derived Met1 Gln774, with a C-terminal 10-His tag. SDS-PAGE: 100-110kD, reducing conditions, Activity: Measured by its ability to produce to produce hydrogen peroxide during the oxidation of benzylamine. Specific Activity: >2pmoles/min/ug, Endotoxin Level: <1EU/1ug (LAL) , Storage and Stability:, Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.