Liver carboxylesterase 1 (CES1), human, recombinant

Organism: Homo sapiens (Human). Source: Baculovirus. Expression Region: 18-567aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: GHPSSPPVVD TVHGKVLGKF VSLEGFAQPV AIFLGIPFAK PPLGPLRFTP PQPAEPWSFV KNATSYPPMC TQDPKAGQLL SELFTNRKEN IPLKLSEDCL YLNIYTPADL TKKNRLPVMV WIHGGGLMVG AASTYDGLAL AAHENVVVVT IQYRLGIWGF FSTGDEHSRG NWGHLDQVAA LRWVQDNIAS FGGNPGSVTI FGESAGGESV SVLVLSPLAK NLFHRAISES GVALTSVLVK KGDVKPLAEQ IAITAGCKTT TSAVMVHCLR QKTEEELLET TLKMKFLSLD LQGDPRESQP LLGTVIDGML LLKTPEELQA ERNFHTVPYM VGINKQEFGW LIPMQLMSYP LSEGQLDQKT AMSLLWKSYP LVCIAKELIP EATEKYLGGT DDTVKKKDLF LDLIADVMFG VPSVIVARNH RDAGAPTYMY EFQYRPSFSS DMKPKTVIGD HGDELFSVFG APFLKEGASE EEIRLSKMVM KFWANFARNG NPNGEGLPHW PEYNQKEGYL QIGANTQAAQ KLKDKEVAFW TNLFAKKAVE KPPQTEHIEL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins. Hydrolyzes cellular cholesteryl esters to free cholesterols and promotes reverse cholesterol transport (RCT) by facilitating both the initial and final steps in the process. First of all, allows free cholesterol efflux from macrophages to extracellular cholesterol acceptors and secondly, releases free cholesterol from lipoprotein-delivered cholesteryl esters in the liver for bile acid synthesis or direct secretion into the bile. Reference: "Inactivation of lipid glyceryl ester metabolism in human THP1 monocytes/macrophages by activated organophosphorus insecticides: role of carboxylesterases 1 and 2." Xie S., Borazjani A., Hatfield M.J., Edwards C.C., Potter P.M., Ross M.K. Chem. Res. Toxicol. 23:1890-1904(2010). Function: nan