LIM and senescent cell antigen-like-containing domain protein 1 (Lims1), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Baculovirus. Expression Region: 2-325aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: ANALASATCE RCKGGFAPAE KIVNSNGELY HEQCFVCAQC FQQFPEGLFY EFEGRKYCEH DFQMLFAPCC HQCGEFIIGR VIKAMNNSWH PECFRCDLCQ EVLADIGFVK NAGRHLCRPC HNREKARGLG KYICQKCHAI IDEQPLIFKN DPYHPDHFNC ANCGKELTAD ARELKGELYC LPCHDKMGVP ICGACRRPIE GRVVNAMGKQ WHVEHFVCAK CEKPFLGHRH YERKGLAYCE THYNQLFGDV CFHCNRVIEG DVVSALNKAW CVSCFACSTC NTKLTLKNKF VEFDMKPVCK KCYEKFPLEL KKRLKKLSET LGRK. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Adapter protein in a cytoplasmic complex linking beta-integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors. Involved in the regulation of cell survival, cell proliferation and cell differentiation. Reference: "PINCH1 plays an essential role in early murine embryonic development but is dispensable in ventricular cardiomyocytes." Liang X., Zhou Q., Li X., Sun Y., Lu M., Dalton N., Ross J. Jr., Chen J. Mol. Cell. Biol. 25:3056-3062(2005). Function: Adapter protein in a cytoplasmic complex linking beta-integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors. Involved in the regulation of cell survival, cell proliferation and cell differentiation.