Leukemia inhibitory factor receptor (LIFR), partial (Active), human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 45-833aa. Protein Length: Partial. Tag Info: C-terminal hFc-Flag-tagged. Target Protein Sequence: QKKGAPHDLK CVTNNLQVWN CSWKAPSGTG RGTDYEVCIE NRSRSCYQLE KTSIKIPALS HGDYEITINS LHDFGSSTSK FTLNEQNVSL IPDTPEILNL SADFSTSTLY LKWNDRGSVF PHRSNVIWEI KVLRKESMEL VKLVTHNTTL NGKDTLHHWS WASDMPLECA IHFVEIRCYI DNLHFSGLEE WSDWSPVKNI SWIPDSQTKV FPQDKVILVG SDITFCCVSQ EKVLSALIGH TNCPLIHLDG ENVAIKIRNI SVSASSGTNV VFTTEDNIFG TVIFAGYPPD TPQQLNCETH DLKEIICSWN PGRVTALVGP RATSYTLVES FSGKYVRLKR AEAPTNESYQ LLFQMLPNQE IYNFTLNAHN PLGRSQSTIL VNITEKVYPH TPTSFKVKDI NSTAVKLSWH LPGNFAKINF LCEIEIKKSN SVQEQRNVTI KGVENSSYLV ALDKLNPYTL YTFRIRCSTE TFWKWSKWSN KKQHLTTEAS PSKGPDTWRE WSSDGKNLII YWKPLPINEA NGKILSYNVS CSSDEETQSL SEIPDPQHKA EIRLDKNDYI ISVVAKNSVG SSPPSKIASM EIPNDDLKIE QVVGMGKGIL LTWHYDPNMT CDYVIKWCNS SRSEPCLMDW RKVPSNSTET VIESDEFRPG IRYNFFLYGC RNQGYQLLRS MIGYIEELAP IVAPNFTVED TSADSILVKW EDIPVEELRG FLRGYLFYFG KGERDTSKMR VLESGRSDIK VKNITDISQK TLRIADLQGK TSYHLVLRAY TDGGVGPEKS MYVVTKENS. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human LIF (CSB-MP012928HU) at 2 µg/ml can bind human LIFR, the EC50 is 11.68-15.52 ng/ml. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: n/a. Function: nan