Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1 (L

Organism: Mus musculus (Mouse). Source: Yeast. Expression Region: 37-555aa. Protein Length: Extracellular Domain. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: PPRCECSAQD RAVLCHRKRF VAVPEGIPTE TRLLDLGKNR IKTLNQDEFA SFPHLEELEL NENIVSAVEP GAFNNLFNLR TLGLRSNRLK LIPLGVFTGL SNLTKLDISE NKIVILLDYM FQDLYNLKSL EVGDNDLVYI SHRAFSGLNS LEQLTLEKCN LTSIPTEALS HLHGLIVLRL RHLNINAIRD YSFKRLYRLK VLEISHWPYL DTMTPNCLYG LNLTSLSITH CNLTAVPYLA VRHLVYLRFL NLSYNPIGTI EGSMLHELLR LQEIQLVGGQ LAVVEPYAFR GLNYLRVLNV SGNQLTTLEE SAFHSVGNLE TLILDSNPLA CDCRLLWVFR RRWRLNFNRQ QPTCATPEFV QGKEFKDFPD VLLPNYFTCR RAHIRDRKAQ QVFVDEGHTV QFVCRADGDP PPAILWLSPR KHLVSAKSNG RLTVFPDGTL EVRYAQVQDN GTYLCIAANA GGNDSMPAHL HVRSYSPDWP HQPNKTFAFI SNQPGEGEAN STRATVPFPF DIKTLIIAT. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation responsible for some inhibition of axonal regeneration by myelin-associated factors. Is also an important negative regulator of oligodentrocyte differentiation and axonal myelination . Acts in conjunction with RTN4 and RTN4R in regulating neuronal precursor cell motility during cortical development.1 Publication. Reference: Nogo-a regulates neural precursor migration in the embryonic mouse cortex.Mathis C., Schroeter A., Thallmair M., Schwab M.E.Cereb. Cortex 20:2380-2390(2010). Function: Functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation responsible for some inhibition of axonal regeneration by myelin-associated factors. Is also an important negative regulator of oligodentrocyte differentiation and axonal myelination (By similarity). Acts in conjunction with RTN4 and RTN4R in regulating neuronal precursor cell motility during cortical development.