Lactosylceramide alpha-2,3-sialyltransferase (ST3GAL5), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 83-418aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: LKLNYTTEEC DMKKMHYVDP DHVKRAQKYA QQVLQKECRP KFAKTSMALL FEHRYSVDLL PFVQKAPKDS EAESKYDPPF GFRKFSSKVQ TLLELLPEHD LPEHLKAKTC RRCVVIGSGG ILHGLELGHT LNQFDVVIRL NSAPVEGYSE HVGNKTTIRM TYPEGAPLSD LEYYSNDLFV AVLFKSVDFN WLQAMVKKET LPFWVRLFFW KQVAEKIPLQ PKHFRILNPV IIKETAFDIL QYSEPQSRFW GRDKNVPTIG VIAVVLATHL CDEVSLAGFG YDLNQPRTPL HYFDSQCMAA MNFQTMHNVT TETKFLLKLV KEGVVKDLSG GIDREF. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc to the non-reducing terminal galactose (Gal) of glycosphingolipids forming gangliosides (important molecules involved in the regulation of multiple cellular processes, including cell proliferation and differentiation, apoptosis, embryogenesis, development, and oncogenesis). Mainly involved in the biosynthesis of ganglioside GM3 but can also use different glycolipids as substrate acceptors such as D-galactosylceramide (GalCer), asialo-GM2 (GA2) and asialo-GM1 (GA1), although less preferentially than beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-Cer (LacCer). Reference: n/a. Function: nan