Lactoperoxidase (LPO), human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 81-712aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: TRTAIRNGQV WEESLKRLRQ KASLTNVTDP SLDLTSLSLE VGCGAPAPVV RCDPCSPYRT ITGDCNNRRK PALGAANRAL ARWLPAEYED GLSLPFGWTP GKTRNGFPLP LAREVSNKIV GYLNEEGVLD QNRSLLFMQW GQIVDHDLDF APDTELGSSE YSKAQCDEYC IQGDNCFPIM FPPNDPKAGT QGKCMPFFRA GFVCPTPPYK SLAREQINAL TSFLDASFVY SSEPSLASRL RNLSSPLGLM AVNQEVSDHG LPYLPYDSKK PSPCEFINTT ARVPCFLAGD SRASEHILLA TSHTLFLREH NRLARELKRL NPQWDGEKLY QEARKILGAF VQIITFRDYL PILLGDHMQK WIPPYQGYSE SVDPRISNVF TFAFRFGHLE VPSSMFRLDE NYQPWGPEPE LPLHTLFFNT WRMVKDGGID PLVRGLLAKK SKLMKQNKMM TGELRNKLFQ PTHRIHGFDL AAINTQRCRD HGQPGYNSWR AFCDLSQPQT LEELNTVLKS KMLAKKLLGL YGTPDNIDIW IGAIAEPLVE RGRVGPLLAC LLGKQFQQIR DGDRFWWENP GVFTNEQKDS LQKMSFSRLV CDNTRITKVP RDPFWANSYP YDFVDCSAID KLDLSPWASV KN. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Antimicrobial agent which utilizes hydrogen peroxide and thiocyanate (SCN) to generate the antimicrobial substance hypothiocyanous acid (HOSCN). May contribute to airway host defense against infection. Reference: "The cytotoxic activity of lactoperoxidase: enhancement and inhibition by neuroactive compounds." Everse J., Coates P.W. Free Radic. Biol. Med. 37:839-849(2004). Function: Antimicrobial agent which utilizes hydrogen peroxide and thiocyanate (SCN) to generate the antimicrobial substance hypothiocyanous acid (HOSCN) (By similarity). May contribute to airway host defense against infection.