L-gulonolactone oxidase (Gulo), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: in vitro E.coli expression system. Expression Region: 2-440aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: VHGYKGVQFQ NWAKTYGCSP EVYYQPTSVE EVREVLALAR EQKKKVKVVG GGHSPSDIAC TDGFMIHMGK MNRVLQVDKE KKQITVEAGI LLADLHPQLD EHGLAMSNLG AVSDVTVAGV IGSGTHNTGI KHGILATQVV ALTLMTADGE VLECSESRNA DVFQAARVHL GCLGIILTVT LQCVPQFHLQ ETSFPSTLKE VLDNLDSHLK RSEYFRFLWF PHTENVSIIY QDHTNKAPSS ASNWFWDYAI GFYLLEFLLW TSTYLPCLVG WINRFFFWML FNCKKESSNL SHKIFTYECR FKQHVQDWAI PREKTKEALL ELKAMLEAHP KVVAHYPVEV RFTRGDDILL SPCFQRDSCY MNIIMYRPYG KDVPRLDYWL AYETIMKKFG GRPHWAKAHN CTQKDFEEMY PTFHKFCDIR EKLDPTGMFL NSYLEKVFY. Purity: Greater than 85% as determined by SDS-PAGE. Protein function: Oxidizes L-gulono-1,4-lactone to hydrogen peroxide and L- xylo-hexulonolactone which spontaneously isomerizes to L-ascorbate. [The UniProt Consortium]. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Oxidizes L-gulono-1,4-lactone to hydrogen peroxide and L-xylo-hexulonolactone which spontaneously isomerizes to L-ascorbate. Reference: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004). Function: nan