L-amino-acid oxidase (Il4i1), recombinant mouse

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 22-630aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: DWKAASSLNP IEKCMEDHDY EQLLKVVTLG LNRTSKPQKV VVVGAGVAGL VAAKMLSDAG HKVTILEADN RIGGRIFTFR DEKTGWIGEL GAMRMPSSHR ILHKLCRTLG LNLTQFTQYD ENTWTEVHNV KLRNYVVEKM PEKLGYNLNN RERGHSPEDI YQMALNKAFK DLKALGCKKA MNKFNKHTLL EYLLEEGNLS RPAVQLLGDV MSEEGFFYLS FAEALRAHAC LSDRLRYSRI VGGWDLLPRA LLSSLSGALL LNAPVVSITQ GRNDVRVHIA TSLHSEKTLT ADVVLLTASG PALQRITFSP PLTRKRQEAL RALHYVAASK VFLSFRRPFW HEEHIEGGHS NTDRPSRLIF YPARGEGSLL LASYTWSDAA APFAGLSTDQ TLRLVLQDVA ALHGPVVFRL WDGRGVVKRW AEDPHSQGGF VVQPPLYGRE AEDYDWSAPF GRIYFAGEHT ALPHGWVETA VKSGLRAAVR INNNYGYGEV DPQMMEHAYA EANYLDQYPE GERPEEQQAR EEVSPDEQEP SHKHLLVETS PEGQQHAFVE AIPELQGHVF VETVPQEKGH AHQNIYPSEH VQVHGEVIPE WHGHGGSGTP QMHRVGDHS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Secreted L-amino-acid oxidase that acts as a key immunoregulator. Has preference for L-aromatic amino acids: converts phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp) to phenylpyruvic acid (PP), hydroxyphenylpyruvic acid (HPP), and indole-3-pyruvic acid (I3P), respectively . Also has weak L-arginine oxidase activity . Reference: n/a. Function: nan