Kinesin-like protein KIF18B (KIF18B), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-355aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MAVEDSTLQV VVRVRPPTPR ELDSQRRPVV QVVDERVLVF NPEEPDGGFP GLKWGGTHDG PKKKGKDLTF VFDRVFGEAA TQQDVFQHTT HSVLDSFLQG YNCSVFAYGA TGAGKTHTML GREGDPGIMY LTTVELYRRL EARQQEKHFE VLISYQEVYN EQIHDLLEPK GPLAIREDPD KGVVVQGLSF HQPASAEQLL EILTRGNRNR TQHPTDANAT SSRSHAIFQI FVKQQDRVPG LTQAVQVAKM SLIDLAGSER ASSTHAKGER LREGANINRS LLALINVLNA LADAKGRKTH VPYRDSKLTR LLKDSLGGNC RTVMIAAISP SSLTYEDTYN TLKYADRAKE IRLSL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: In complex with KIF2C, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Its major role may be to transport KIF2C and/or MAPRE1 along microtubules. Reference: "A complex of Kif18b and MCAK promotes microtubule depolymerization and is negatively regulated by Aurora kinases." Tanenbaum M.E., Macurek L., van der Vaart B., Galli M., Akhmanova A., Medema R.H. Curr. Biol. 21:1356-1365(2011). Function: nan