Kelch repeat and BTB domain-containing protein 7 (KBTBD7), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-684aa. Protein Length: Full Length. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: MQSREDVPRS RRLASPRGGR RPKRISKPSV SAFFTGPEEL KDTAHSAALL AQLKSFYDAR LLCDVTIEVV TPGSGPGTGR LFSCNRNVLA AACPYFKSMF TGGMYESQQA SVTMHDVDAE SFEVLVDYCY TGRVSLSEAN VQRLYAASDM LQLEYVREAC ASFLARRLDL TNCTAILKFA DAFDHHKLRS QAQSYIAHNF KQLSRMGSIR EETLADLTLA QLLAVLRLDS LDIESERTVC HVAVQWLEAA AKERGPSAAE VFKCVRWMHF TEEDQDYLEG LLTKPIVKKY CLDVIEGALQ MRYGDLLYKS LVPVPNSSSS SSSSNSLVSA AENPPQRLGM CAKEMVIFFG HPRDPFLCYD PYSGDIYTMP SPLTSFAHTK TVTSSAVCVS PDHDIYLAAQ PRKDLWVYKP AQNSWQQLAD RLLCREGMDV AYLNGYIYIL GGRDPITGVK LKEVECYSVQ RNQWALVAPV PHSFYSFELI VVQNYLYAVN SKRMLCYDPS HNMWLNCASL KRSDFQEACV FNDEIYCICD IPVMKVYNPA RGEWRRISNI PLDSETHNYQ IVNHDQKLLL ITSTTPQWKK NRVTVYEYDT REDQWINIGT MLGLLQFDSG FICLCARVYP SCLEPGQSFI TEEDDARSES STEWDLDGFS ELDSESGSSS SFSDDEVWVQ VAPQRNAQDQ QGSL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: Not Test. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: As part of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex functions as a substrate adapter for the RAC1 guanine exchange factor (GEF) TIAM1, mediating its 'Lys-48' ubiquitination and proteasomal degradation. By controlling this ubiquitination, regulates RAC1 signal transduction and downstream biological processes including the organization of the cytoskeleton, cell migration and cell proliferation. Ubiquitination of TIAM1 requires the membrane-associated protein GABARAP which may restrict locally the activity of the complex. Reference: n/a. Function: nan