Kallikrein-7 (KLK7), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 28-253aa. Protein Length: Partial. Tag Info: N-terminal GST-tagged. Target Protein Sequence: DKIIDGAPCA RGSHPWQVAL LSGNQLHCGG VLVNERWVLT AAHCKMNEYT VHLGSDTLGD RRAQRIKASK SFRHPGYSTQ THVNDLMLVK LNSQARLSSM VKKVRLPSRC EPPGTTCTVS GWGTTTSPDV TFPSDLMCVD VKLISPQDCT KVYKDLLENS MLCAGIPDSK KNACNGDSGG PLVCRGTLQG LVSWGTFPCG QPNDPGVYTQ VCKFTKWIND TMKKHR. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-, -Gln-15' and insulin B chain at '6-Leu-, -Cys-7', '16-Tyr-, -Leu-17', '25-Phe-, -Tyr-26' and '26-Tyr-, -Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines. Reference: Vaspin inhibits kallikrein 7 by serpin mechanism.Heiker J.T., Kloting N., Kovacs P., Kuettner E.B., Strater N., Schultz S., Kern M., Stumvoll M., Bluher M., Beck-Sickinger A.G.Cell. Mol. Life Sci. 70:2569-2583(2013). Function: May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-, -Gln-15' and insulin B chain at '6-Leu-, -Cys-7', '16-Tyr-, -Leu-17', '25-Phe-, -Tyr-26' and '26-Tyr-, -Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.