Izumo sperm-egg fusion protein 1 (IZUMO1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 22-292aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: CVICDPSVVL ALKSLEKDYL PGHLDAKHHK AMMERVENAV KDFQELSLNE DAYMGVVDEA TLQKGSWSLL KDLKRITDSD VKGDLFVKEL FWMLHLQKET FATYVARFQK EAYCPNKCGV MLQTLIWCKN CKKEVHACRK SYDCGERNVE VPQMEDMILD CELNWHQASE GLTDYSFYRV WGNNTETLVS KGKEATLTKP MVGPEDAGSY RCELGSVNSS PATIINFHVT VLPKMIKEEK PSPNIVTPGE ATTESSISLQ PLQPEKMLAS R. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion . The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Reference: "The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs." Inoue N., Ikawa M., Isotani A., Okabe M. Nature 434:234-238(2005). Function: nan