Interleukin-31 receptor subunit alpha (Il31ra), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 19-499aa. Protein Length: Extracellular Domain. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: VLPTKPENIS CVFYFDRNLT CTWRPEKETN DTSYIVTLTY SYGKSNYSDN ATEASYSFPR SCAMPPDICS VEVQAQNGDG KVKSDITYWH LISIAKTEPP IILSVNPICN RMFQIQWKPR EKTRGFPLVC MLRFRTVNSS HWTEVNFENC KQVCNLTGLQ AFTEYVLALR FRFNDSRYWS KWSKEETRVT MEEVPHVLDL WRILEPADMN GDRKVRLLWK KARGAPVLEK TFGYHIQYFA ENSTNLTEIN NITTQQYELL LMSQAHSVSV TSFNSLGKSQ EAILRIPDVH EKTFQYIKSM KAYIAEPLLV VNWQSSIPAV DTWIVEWLPE AAMSKFPALS WESVSQVTNW TIEQDKLKPF TCYNISVYPV LGHRVGEPYS IQAYAKEGTP LKGPETRVEN IGLRTATITW KEIPKSARNG FINNYTVFYQ AEGGKELSKT VNSHALQCDL ESLTRRTSYT VWVMASTRAG GTNGVRINFK T. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Associates with OSMR to form the interleukin-31 receptor which activates STAT3 and to a lower extent STAT1 and STAT5. May function in skin immunity. Mediates IL31-induced itch, probably in a manner dependent on cation channels TRPA1 and TRPV1. Positively regulates numbers and cycling status of immature subsets of myeloid progenitor cells in bone marrow in vivo and enhances myeloid progenitor cell survival in vitro. Reference: "A novel type I cytokine receptor is expressed on monocytes, signals proliferation, and activates STAT-3 and STAT-5." Ghilardi N., Li J., Hongo J.-A., Yi S., Gurney A., De Sauvage F.J. J. Biol. Chem. 277:16831-16836(2002). Function: Associates with OSMR to form the interleukin-31 receptor which activates STAT3 and to a lower extent STAT1 and STAT5. May function in skin immunity