Interleukin-15 receptor subunit alpha (Il15ra), partial (Active), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 33-205aa. Protein Length: Partial. Tag Info: C-terminal Fc-tagged. Target Protein Sequence: GTTCPPPVSI EHADIRVKNY SVNSRERYVC NSGFKRKAGT STLIECVINK NTNVAHWTTP SLKCIRDPSL AHYSPVPTVV TPKVTSQPES PSPSAKEPEA FSPKSDTAMT TETAIMPGSR LTPSQTTSAG TTGTGSHKSS RAPSLAATMT LEPTASTSLR ITEISPHSSK MTK. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/µg as determined by LAL method. Biological Activity: The ED50 as determined by its ability to block human IL-15-induced proliferation of CTLL-2 mouse cytotoxic T cells is 0.5-2 ng/ml. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM PB, 150 mM NaCl, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Mouse interleukin-15 receptor subunit alpha, also known as Il15ra, is a high-affinity receptor for interleukin-15. Il15ra associates as a heterotrimer with the IL-2 receptor beta and gamma subunits (Common gamma chain, or gamma c) to initiate signal transduction. It can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Il15ra is expressed in special cells including a wide variety of Tand B cells and non-lymphoid cells. Human Il15ra shares 45% amino acid sequence homology with the mouse form of the receptor. Eight isoforms of IL-15 R alpha mRNA have been identified, resulting from alternative splicing events involving different exons. Reference: n/a. Function: High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Signal transduction involves SYK (By similarity).