Interleukin-10 receptor subunit alpha (IL10RA) (3-6: PCLV->GCVL)-VLPs, recombinant human

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 22-578aa(3-6:PCLV->GCVL). Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: HGTELPSPPS VWFEAEFFHH ILHWTPIPNQ SESTCYEVAL LRYGIESWNS ISNCSQTLSY DLTAVTLDLY HSNGYRARVR AVDGSRHSNW TVTNTRFSVD EVTLTVGSVN LEIHNGFILG KIQLPRPKMA PANDTYESIF SHFREYEIAI RKVPGNFTFT HKKVKHENFS LLTSGEVGEF CVQVKPSVAS RSNKGMWSKE ECISLTRQYF TVTNVIIFFA FVLLLSGALA YCLALQLYVR RRKKLPSVLL FKKPSPFIFI SQRPSPETQD TIHPLDEEAF LKVSPELKNL DLHGSTDSGF GSTKPSLQTE EPQFLLPDPH PQADRTLGNR EPPVLGDSCS SGSSNSTDSG ICLQEPSLSP STGPTWEQQV GSNSRGQDDS GIDLVQNSEG RAGDTQGGSA LGHHSPPEPE VPGEEDPAAV AFQGYLRQTR CAEEKATKTG CLEEESPLTD GLGPKFGRCL VDEAGLHPPA LAKGYLKQDP LEMTLASSGA PTGQWNQPTE EWSLLALSSC SDLGISDWSF AHDLAPLGCV AAPGGLLGSF NSDLVTLPLI SSLQSSE. Purity: The purity information is not available for VLPs proteins. Endotoxin: Not test. Biological Activity: n/a. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80°C. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20°C/-80°C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant). Relevance: Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well (PubMed:16982608). In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively (PubMed:12133952). These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy (PubMed:26962683). Reference: n/a. Function: nan