Interleukin-1 receptor-associated kinase 3 (Irak3), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Baculovirus. Expression Region: 1-609aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MAGRCGARGA LSPQLLLFDL PPALLGELCG ILDSCDGPLG WRGLAERLSN SWLDVRHIEK YVNQGKSGTR ELLWSWAQKN KTIGDLLEVL QDMGHQRAIH LIINYGVSWT PSVQTHHELP FPSFPPEVKH ACRENDPGPL EPANVTVDNV LVPEHNEKGT LQKTPISFQS ILEGTKHFHK DFLIGEGEIF EVYRVDIRNQ AYAVKLFKQE KKMQLKKHWK RFLSELEVLL LFRHPHILEL AAYFTETEKL CLVYPYMSNG TLFDRLQCTN GTTPLSWHVR ISVLIGIAKA IQYLHNTQPC AVICGNVSSA NILLDDQLQP KLTDFAAAHF RPNLEQQSST INMTGGGRKH LWYMPEEYIR QGRLSVKTDV YSFGIVIMEV LTGCKVVLDD PKHVQLRDLL MELMEKRGLD SCLSFLDRKI PPCPRNFSAK LFSLAGRCVA TKAKLRPTMD EVLSSLESTQ PSLYFAEDPP TSLKSFRCPS PLFLDNVPSI PVEDDENQNN HSVPPKEVLG TDRVTQKTPF ECSQSEVTFL GLDRNRGNRG SEADCNVPSS SHEECWSPEL VAPSQDLSPT VISLGSSWEV PGHSYGSKPM EKRCSSGLFC SEHEQSKKQ. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex. Reference: "Identification and characterization of murine IRAK-M." Rosati O., Martin M.U. Biochem. Biophys. Res. Commun. 293:1472-1477(2002). Function: nan