Inositol hexakisphosphate kinase 1 (IP6K1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-441aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MCVCQTMEVG QYGKNASRAG DRGVLLEPFI HQVGGHSSMM RYDDHTVCKP LISREQRFYE SLPPEMKEFT PEYKGVVSVC FEGDSDGYIN LVAYPYVESE TVEQDDTTER EQPRRKHSRR SLHRSGSGSD HKEEKASLSL ETSESSQEAK SPKVELHSHS EVPFQMLDGN SGLSSEKISH NPWSLRCHKQ QLSRMRSESK DRKLYKFLLL ENVVHHFKYP CVLDLKMGTR QHGDDASAEK AARQMRKCEQ STSATLGVRV CGMQVYQLDT GHYLCRNKYY GRGLSIEGFR NALYQYLHNG LDLRRDLFEP ILSKLRGLKA VLERQASYRF YSSSLLVIYD GKECRAESCL DRRSEMRLKH LDMVLPEVAS SCGPSTSPSN TSPEAGPSSQ PKVDVRMIDF AHSTFKGFRD DPTVHDGPDR GYVFGLENLI SIMEQMRDEN Q. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Converts inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). Converts 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4. Reference: "Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain." Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y., Ohara O., Tanaka A., Kotani H., Miyajima N., Nomura N. DNA Res. 3:321-329(1996). Function: Converts inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). Converts 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4.