Importin subunit alpha-1 (KPNA2), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-529aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: STNENANTPA ARLHRFKNKG KDSTEMRRRR IEVNVELRKA KKDDQMLKRR NVSSFPDDAT SPLQENRNNQ GTVNWSVDDI VKGINSSNVE NQLQATQAAR KLLSREKQPP IDNIIRAGLI PKFVSFLGRT DCSPIQFESA WALTNIASGT SEQTKAVVDG GAIPAFISLL ASPHAHISEQ AVWALGNIAG DGSVFRDLVI KYGAVDPLLA LLAVPDMSSL ACGYLRNLTW TLSNLCRNKN PAPPIDAVEQ ILPTLVRLLH HDDPEVLADT CWAISYLTDG PNERIGMVVK TGVVPQLVKL LGASELPIVT PALRAIGNIV TGTDEQTQVV IDAGALAVFP SLLTNPKTNI QKEATWTMSN ITAGRQDQIQ QVVNHGLVPF LVSVLSKADF KTQKEAVWAV TNYTSGGTVE QIVYLVHCGI IEPLMNLLTA KDTKIILVIL DAISNIFQAA EKLGETEKLS IMIEECGGLD KIEALQNHEN ESVYKASLSL IEKYFSVEEE EDQNVVPETT SEGYTFQVQD GAPGTFNF. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the irreversible reduction of the cytotoxic compound methylglyoxal (MG, 2-oxopropanal) to (S)-lactaldehyde as an alternative to detoxification of MG by glyoxalase I GLO1. MG is synthesized via a bypath of glycolysis from dihydroxyacetone phosphate and is believed to play a role in cell cycle regulation and stress adaptation. Also catalyzes the reduction of 3-methylbutanal to 3-methylbutanol. Acts as a suppressor of 3-methylbutanol-induced filamentation by modulating the levels of 3-methylbutanal, the signal to which cells respond by filamentation. Also involved in ergosterol metabolism. Reference: "Structural insights into the cofactor-assisted substrate recognition of yeast methylglyoxal/isovaleraldehyde reductase Gre2." Guo P.C., Bao Z.Z., Ma X.X., Xia Q., Li W.F. Biochim. Biophys. Acta 1844:1486-1492(2014). Function: nan