Immunoglobulin superfamily member 2 (Cd101), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: Baculovirus. Expression Region: 22-266aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: REVKIQEGPL YRAEGYPVSI RCTVSGHQGP STQDFRWSIY LPSAPTKEVQ IISTKDAGFS YAVYAQRVQS KEIYIERLQG DSVLLHISKL QMKDAGEYEC HTPNTDGKYF GSYSAKTNLT VVPDTLSATM PSQTLSKKEG EPLELTCETT KATVQHTHLS LTWYLMQEGG GSQATEIVSL SKDFVLTPGS SYADRFVAGD VRLDKLGATS FRLSVGKLQP SDQGQVFCEA TEWIQDPDET WTLIT. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Plays a role as inhibitor of T-cells proliferation induced by CD3. Inhibits expression of IL2RA on activated T-cells and secretion of IL2. Inhibits tyrosine kinases that are required for IL2 production and cellular proliferation. Inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3-induced changes in intracellular free calcium. Prevents nuclear translocation of nuclear factor of activated T-cell to the nucleus. Plays a role in the inhibition of T-cell proliferation via IL10 secretion by cutaneous dendritic cells. Reference: "Identification of Cd101 as a susceptibility gene for Novosphingobium aromaticivorans-induced liver autoimmunity." Mohammed J.P., Fusakio M.E., Rainbow D.B., Moule C., Fraser H.I., Clark J., Todd J.A., Peterson L.B., Savage P.B., Wills-Karp M., Ridgway W.M., Wicker L.S., Mattner J. J. Immunol. 187:337-349(2011). Function: Plays a role as inhibitor of T-cells proliferation induced by CD3. Inhibits expression of IL2RA on activated T-cells and secretion of IL2. Inhibits tyrosine kinases that are required for IL2 production and cellular proliferation. Inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3-induced changes in intracellular free calcium. Prevents nuclear translocation of nuclear factor of activated T-cell to the nucleus. Plays a role in the inhibition of T-cell proliferation via IL10 secretion by cutaneous dendritic cells (By similarity).