Hyaluronan synthase 1 (has1), partial, Xenopus tropicalis, recombinant

Organism: Xenopus tropicalis (Western clawed frog) (Silurana tropicalis). Source: E.coli. Expression Region: 82-401aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: AYLEIRRINK TDLPCSFKKT VALTIAGYQE NPDYLKHCLD SCRYVKYPKD KLKIILVIDG NTEDDAYMME MFKDVFHGDD VGTYVWKGNY HTGVKETQDG SCPEVSKPLN EDEGIRIVEE LVRTKRCVCI MQQWGGKREV MYTAFRAIGT TMDYVQVCDS DTKLDELATV EMVKVLEANE LCGAVGGDVR ILNPYDSFIS FMSSLRYWMA FNVERACQSY FDCVSCISGP LGMYRNDILQ VFLEAWHSQK FLGTYCTLGD DRHLTNRVLS MGYRTKYTPK CRAFSETPSQ YLRWLNQQTR WTKSYFREWL YNAQWWYKHH. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate. Reference: NIH - Xenopus Gene Collection (XGC) project Submitted (APR-2008). Function: nan