Histidine--tRNA ligase, cytoplasmic (HARS1),partial, human, recombinant

Organism: Homo sapiens (Human). Source: Baculovirus. Expression Region: 2-504aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: AERAALEELV KLQGERVRGL KQQKASAELI EEEVAKLLKL KAQLGPDESK QKFVLKTPKG TRDYSPRQMA VREKVFDVII RCFKRHGAEV IDTPVFELKE TLMGKYGEDS KLIYDLKDQG GELLSLRYDL TVPFARYLAM NKLTNIKRYH IAKVYRRDNP AMTRGRYREF YQCDFDIAGN FDPMIPDAEC LKIMCEILSS LQIGDFLVKV NDRRILDGMF AICGVSDSKF RTICSSVDKL DKVSWEEVKN EMVGEKGLAP EVADRIGDYV QQHGGVSLVE QLLQDPKLSQ NKQALEGLGD LKLLFEYLTL FGIDDKISFD LSLARGLDYY TGVIYEAVLL QTPAQAGEEP LGVGSVAAGG RYDGLVGMFD PKGRKVPCVG LSIGVERIFS IVEQRLEALE EKIRTTETQV LVASAQKKLL EERLKLVSEL WDAGIKAELL YKKNPKLLNQ LQYCEEAGIP LVAIIGEQEL KDGVIKLRSV TSREEVDVRR EDLVEEIKRR TGQ. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins.The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. Reference: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.Bian Y., Song C., Cheng K., Dong M., Wang F.,Huang J., Sun D., Wang L., Ye M., Zou H.J. Proteomics 96:253-262(2014). Function: Cytoplasmic histidine--tRNA ligase (Probable). Plays a role in axon guidance.