High affinity immunoglobulin gamma Fc receptor I (FCGR1A), human, recombinant

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 16-374aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: QVDTTKAVIT LQPPWVSVFQ EETVTLHCEV LHLPGSSSTQ WFLNGTATQT STPSYRITSA SVNDSGEYRC QRGLSGRSDP IQLEIHRGWL LLQVSSRVFT EGEPLALRCH AWKDKLVYNV LYYRNGKAFK FFHWNSNLTI LKTNISHNGT YHCSGMGKHR YTSAGISVTV KELFPAPVLN ASVTSPLLEG NLVTLSCETK LLLQRPGLQL YFSFYMGSKT LRGRNTSSEY QILTARREDS GLYWCEAATE DGNVLKRSPE LELQVLGLQL PTPVWFHVLF YLAVGIMFLV NTVLWVTIRK ELKRKKKWDL EISLDSGHEK KVISSLQEDR HLEEELKCQE QKEEQLQEGV HRKEPQGAT. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Mediates IgG effector functions on monocytes triggering antibody-dependent cellular cytotoxicity (ADCC) of virus-infected cells. Reference: "Crystal structure of Fcgamma receptor I and its implication in high affinity gamma-immunoglobulin binding." Lu J., Ellsworth J.L., Hamacher N., Oak S.W., Sun P.D. J. Biol. Chem. 286:40608-40613(2011). Function: nan