Heterogeneous nuclear ribonucleoprotein M (HNRNPM), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-730aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: AAGVEAAAEV AATEIKMEEE SGAPGVPSGN GAPGPKGEGE RPAQNEKRKE KNIKRGGNRF EPYANPTKRY RAFITNIPFD VKWQSLKDLV KEKVGEVTYV ELLMDAEGKS RGCAVVEFKM EESMKKAAEV LNKHSLSGRP LKVKEDPDGE HARRAMQKVM ATTGGMGMGP GGPGMITIPP SILNNPNIPN EIIHALQAGR LGSTVFVANL DYKVGWKKLK EVFSMAGVVV RADILEDKDG KSRGIGTVTF EQSIEAVQAI SMFNGQLLFD RPMHVKMDER ALPKGDFFPP ERPQQLPHGL GGIGMGLGPG GQPIDANHLN KGIGMGNIGP AGMGMEGIGF GINKMGGMEG PFGGGMENMG RFGSGMNMGR INEILSNALK RGEIIAKQGG GGGGGSVPGI ERMGPGIDRL GGAGMERMGA GLGHGMDRVG SEIERMGLVM DRMGSVERMG SGIERMGPLG LDHMASSIER MGQTMERIGS GVERMGAGMG FGLERMAAPI DRVGQTIERM GSGVERMGPA IERMGLSMER MVPAGMGAGL ERMGPVMDRM ATGLERMGAN NLERMGLERM GANSLERMGL ERMGANSLER MGPAMGPALG AGIERMGLAM GGGGGASFDR AIEMERGNFG GSFAGSFGGA GGHAPGVARK ACQIFVRNLP FDFTWKMLKD KFNECGHVLY ADIKMENGKS KGCGVVKFES PEVAERACRM MNGMKLSGRE IDVRIDRNA. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Pre-mRNA binding protein in vivo, binds avidly to poly and poly RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines. Reference: "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis." Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J. RNA 8:426-439(2002). Function: nan