Heterogeneous nuclear ribonucleoprotein H (HNRNPH1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-449aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MLGTEGGEGF VVKVRGLPWS CSADEVQRFF SDCKIQNGAQ GIRFIYTREG RPSGEAFVEL ESEDEVKLAL KKDRETMGHR YVEVFKSNNV EMDWVLKHTG PNSPDTANDG FVRLRGLPFG CSKEEIVQFF SGLEIVPNGI TLPVDFQGRS TGEAFVQFAS QEIAEKALKK HKERIGHRYI EIFKSSRAEV RTHYDPPRKL MAMQRPGPYD RPGAGRGYNS IGRGAGFERM RRGAYGGGYG GYDDYNGYND GYGFGSDRFG RDLNYCFSGM SDHRYGDGGS TFQSTTGHCV HMRGLPYRAT ENDIYNFFSP LNPVRVHIEI GPDGRVTGEA DVEFATHEDA VAAMSKDKAN MQHRYVELFL NSTAGASGGA YEHRYVELFL NSTAGASGGA YGSQMMGGMG LSNQSSYGGP ASQQLSGGYG GGYGGQSSMS GYDQVLQENS SDFQSNIA. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG). Reference: Heterogeneous nuclear ribonucleoproteins H, H', and F are members of a ubiquitously expressed subfamily of related but distinct proteins encoded by genes mapping to different chromosomes.Honore B., Rasmussen H.H., Vorum H., Dejgaard K., Liu X., Gromov P., Madsen P., Gesser B., Tommerup N., Celis J.E.J. Biol. Chem. 270:28780-28789(1995). Function: This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG).