Hepatitis C Virus Core, 22kD, Recombinant (HCV)

Hepatitis C full length nucleocapsid recombinant proretn (truncated p22) produced in Yeast expression system. Amino Acid Sequence: 1 mstnpkpqrk tkrntnrrpq dvkfpgggqi vggvyllprr gprlgvratr ktsersqprg, 61 rrqpipkarr pegrtwaqpg ypwplygneg cgwagwllsp rgsrpswgpt dprrrsrnlg, 121 kvidtltcgf adlmgyiplv gaplggaara lahgvrvled gvnyatgnlp gcsfsiflla, 181 llscltvpas a, Applications: Suitable for use in ELISA, Western Blot, solid phase antigen-antibody titration assay and T-Cell Activation. Other applications not tested. Recommended Dilutions: solid phase antigen-antibody titration assay:, Dilute the antigen preparation 10 to 20-fold in PBS., Western Blot: 10ng range depending on the nature and affinity of the secondary detection reagent. , ELISA: HCV converted human serum polyclonal antibodies yield titers of 1:10, 000 or greater at 100ng of immobilized protein under standard ELISA conditions., Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.