Heparin lyase 21A, Bacteroides stercoris

Heparin lyase 21A (BsHep21A), assigned the E.C. number 4.2.2.8 and 4.2.2.7 , is a derivative of Bacteroides stercoris. It is a bifunctional enzyme that acts on linkages between acetyl-glucosamine and uronate of polysaccharides containing glucuronate or iduronate residues and 1,4-alpha linked 2-sulfoamino-2-deoxy-6-sulfo-D-glucose residues. The recombinant BsHep21A, purified from Escherichia coli, is a single-domain Pectate Lyase family 21 (PL21) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Polysaccharide Lyase. Activity: Heparin lyases. Family: PL21. Ec Number: 4.2.2.8 and 4.2.2.7. Organism: Bacteroides stercoris. Architecture: PL21. Optimal pH: 7.4. Optimal Temperature: 32°C. Substrates: acharan sulfate