Heparin lyase 15A, Bacteroides thetaiotaomicron

Heparin lyase 15A (BtHep15A), assigned the E.C. number 4.2.2.-, is a derivative of Bacteroides thetaiotaomicron. It is an exo-processive lyase that degrades oligosaccharides resulting from the degradation of heparin and heparan sulfate. The recombinant BtHep15A, purified from Escherichia coli, is a single-domain Pectate Lyase family 15 (PL15) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Polysaccharide Lyase. Activity: Heparin lyases. Family: PL15. Ec Number: 4.2.2.-. Organism: Bacteroides thetaiotaomicron. Architecture: PL15. Optimal pH: 6.5. Optimal Temperature: 37°C. Substrates: heparan sulfate, heparin