Hemopexin (Hpx), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: Yeast. Expression Region: 24-460aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: NPLPAAHETV AKGENGTKPD SDVIEHCSDA WSFDATTMDH NGTMLFFKGE FVWRGHSGIR ELISERWKNP VTSVDAAFRG PDSVFLIKED KVWVYPPEKK ENGYPKLFQE EFPGIPYPPD AAVECHRGEC QSEGVLFFQG NRKWFWDFAT RTQKERSWPA VGNCTAALRW LERYYCFQGN KFLRFNPVTG EVPPRYPLDA RDYFISCPGR GHGKLRNGTA HGNSTHPMHS RCNADPGLSA LLSDHRGATY AFSGSHYWRL DSSRDGWHSW PIAHHWPQGP SAVDAAFSWD EKVYLIQGTQ VYVFLTKGGN NLVSGYPKRL EKELGSPPGI SLDTIDAAFS CPGSSKLYVT SGRRLWWLDL KSGAQATWAE LSWPHEKVDG ALCLEKSLGP YSCSSNGPNL FFIHGPNLYC YSSIDKLNAA KSLPQPQKVN SILGCSQ. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Binds he and transports it to the liver for breakdown and iron recovery, after which the free hopexin returns to the circulation. Reference: Rat hemopexin. Molecular cloning, primary structural characterization, and analysis of gene expression.Nikkilae H., Gitlin J.D., Mueller-Eberhard U.Biochemistry 30:823-829(1991). Function: Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.