Hemagglutinin-neuraminidase (HN), partial, human parainfluenza 1 virus, recombinant

Organism: Human parainfluenza 1 virus (strain Washington/1957) (HPIV-1). Source: E.coli. Expression Region: 454-575aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: VLSRPGNQDC NWYNRCPREC ISGVYTDAYP LSPDAVNVAT TTLYANTSRV NPTIMYSNTS EIINMLRLKN VQLEAAYTTT SCITHFGKGY CFHIVEINQT SLNTLQPMLF KTSIPKICKI TS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion . Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins. Reference: "The hemagglutinin-neuraminidase glycoproteins of human parainfluenza virus type 1 and Sendai virus have high structure-function similarity with limited antigenic cross-reactivity." Gorman W.L., Gill D.S., Scroggs R.A., Portner A. Virology 175:211-221(1990). Function: nan