Heat shock protein 21, chloroplastic (HSP21), Arabidopsis thaliana, recombinant

Organism: Arabidopsis thaliana (Mouse-ear cress). Source: E.coli. Expression Region: 44-227aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: AQDQRENSID VVQQGQQKGN QGSSVEKRPQ QRLTMDVSPF GLLDPLSPMR TMRQMLDTMD RMFEDTMPVS GRNRGGSGVS EIRAPWDIKE EEHEIKMRFD MPGLSKEDVK ISVEDNVLVI KGEQKKEDSD DSWSGRSVSS YGTRLQLPDN CEKDKIKAEL KNGVLFITIP KTKVERKVID VQIQ. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Chaperone protein required for seedling and chloroplast development under heat stress, probably by maintaining plastid-encoded RNA polymerase (PEP)-dependent transcription. Reference: "Chloroplast small heat shock protein HSP21 interacts with plastid nucleoid protein pTAC5 and is essential for chloroplast development in Arabidopsis under heat stress." Zhong L., Zhou W., Wang H., Ding S., Lu Q., Wen X., Peng L., Zhang L., Lu C. Plant Cell 25:2925-2943(2013). Function: nan