GRB10-interacting GYF protein 2 (GIGYF2), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 510-1030aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: ERLASKLQEH RAKGVSIPLM HEAMQKWYYK DPQGEIQGPF NNQEMAEWFQ AGYFTMSLLV KRACDESFQP LGDIMKMWGR VPFSPGPAPP PHMGELDQER LTRQQELTAL YQMQHLQYQQ FLIQQQYAQV LAQQQKAALS SQQQQQLALL LQQFQTLKMR ISDQNIIPSV TRSVSVPDTG SIWELQPTAS QPTVWEGGSV WDLPLDTTTP GPALEQLQQL EKAKAAKLEQ ERREAEMRAK REEEERKRQE ELRRQQEEIL RRQQEEERKR REEEELARRK QEEALRRQRE QEIALRRQRE EEERQQQEEA LRRLEERRRE EEERRKQEEL LRKQEEEAAK WAREEEEAQR RLEENRLRME EEAARLRHEE EERKRKELEV QRQKELMRQR QQQQEALRRL QQQQQQQQLA QMKLPSSSTW GQQSNTTACQ SQATLSLAEI QKLEEERERQ LREEQRRQQR ELMKALQQQQ QQQQQKLSGW GNVSKPSGTT KSLLEIQQEE ARQMQKQQQQ QQQHQQPNRA R. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Key component of the 4EHP-GYF2 complex, a multiprotein complex that acts as a repressor of translation initiation. In the 4EHP-GYF2 complex, acts as a factor that bridges EIF4E2 to ZFP36/TTP, linking translation repression with mRNA decay. Also recruits and bridges the association of the 4EHP complex with the decapping effector protein DDX6, which is required for the ZFP36/TTP-mediated down-regulation of AU-rich mRNA. May act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling, including IGF1 and insulin receptors. Reference: n/a. Function: nan