Granzyme K (Gzmk), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 26-263aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: IIGGREVQPH SRPFMASIQY RSKHICGGVL IHPQWVLTAA HCYSWFPRGH SPTVVLGAHS LSKNEPMKQT FEIKKFIPFS RLQSGSASHD IMLIKLRTAA ELNKNVQLLH LGSKNYLRDG TKCQVTGWGT TKPDLLTASD TLREVTVTII SRKRCNSQSY YNHKPVITKD MICAGDARGQ KDSCKGDSGG PLICKGIFHA LVSQGYKCGI AKKPGIYTLL TKKYQTWIKS KLAPSRAH. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: "Granzyme K directly processes bid to release cytochrome c and endonuclease G leading to mitochondria-dependent cell death." Zhao T., Zhang H., Guo Y., Fan Z. J Biol Chem 282:12104-12111(2007). Function: nan