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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
G8235-58H.50 | 50 µg | - | - |
3 - 19 business days* |
952.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
The Glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked heparan... more
Product information "Glypican 6, Recombinant, Human (Glypiated Proteoglycan 6)"
The Glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked heparan sulfate (HS) proteoglycans that likely play a key role in embryonic morphogenesis (1, 2, 3, 4). There are currently six known mammalian Glypicans. They all share a common-sized protein core of 60-70kD, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of Glypicans are known, with one subfamily containing Glypicans 1, 2, 4 and 6, and another subfamily containing Glypicans 3 and 5 (3, 5). Human Glypican 6 (GPC-6) is synthesized as a 554 amino acid (aa) preproprecursor that contains a 23 aa signal sequence, a 505 aa mature region and a 26 aa C-terminal prosegment (5, 6). There are four consecutive Ser-Gly repeats that serve as a heparin sulfate attachment site. GPC-6 is reported to be as large as 110kD in size. This translates into approximately 50kD of proteoglycan (5). Human to mouse, there is 97% aa identity over the entire GPC-6 molecule. Cells known to express GPC-6 are adult ovary and embryonic vascular and visceral smooth muscle, plus mesenchyme (embryonic connective tissue) in multiple organs (1, 5, 6). The function of GPC-6 is essentially unknown. As a Glypican family member, it may facilitate heparin-binding growth factor signaling and polyamine uptake into expressing cells (7, 8). In this regard, it would appear that GPC-6 with its attendant HS is downregulated by triiodothyronine during cartilage maturation, thus limiting the availability of sites for FGF sequestration and activity (9). Source: DNA sequence encoding the mature human Glypican 6 (Asp 24-Val 527, Accession # Q9Y625) was fused with six Histidines at the C-terminus and the protein was expressed in a mouse myeloma cell line, NS0. , Definition: Measured by its ability to bind biotinylated rmWnt-3A captured on streptavidin-coated plate. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 38kD and 20kD, respectively migrates at ~ 38 and 27-29kD under reducing conditions. , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: | GPC6, Glypican-6, Secreted glypican-6 |
Supplier: | United States Biological |
Supplier-Nr: | G8235-58H |
Properties
Application: | WB |
Conjugate: | No |
Species reactivity: | human |
Format: | Highly Purified |
Database Information
KEGG ID : | K08112 | Matching products |
UniProt ID : | Q9Y625 | Matching products |
Gene ID | GeneID 10082 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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