Glypican-3 (Gpc3), partial, rat, recombinant

Organism: Rattus norvegicus (Rat). Source: Yeast. Expression Region: 25-560aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: QPPPPPDATC HQVRSFFQRL QPGLKWVPET PVPGSDLQVC LPKGPTCCSR KMEEKYQLTA RLNMEQLLQS ASMELKFLII QNAAVFQEAF EIVVRHAKNY TNAMFKNNYP SLTPQAFEFV GEFFTDVSLY ILGSDINVDD MVNELFDSLF PVIYTQMMNP GLPESVLDIN ECLRGARRDL KVFGSFPKLI MTQVSKSLQV TRIFLQALNL GIEVINTTDH LKFSKDCGRM LTRMWYCSYC QGLMMVKPCG GYCNVVMQGC MAGVVEIDKY WREYILSLEE LVNGMYRIYD MENVLLGLFS TIHDSIQYVQ KNGGKLTTTI GKLCAHSQQR QYRSAYYPED LFIDKKVLKV ARVEHEETLS SRRRELIQKL KSFISFYSAL PGYICSHSPV AENDTLCWNG QELVERYSQK AARNGMKNQF NLHELKMKGP EPVVSQIIDK LKHINQLLRT MSVPKGKVVD KSLDEEGLES GDCGDDEDEC IGSSGDGMMK VKNQLRFLAE LAYDLDVDDA PGNKQHGNQK DNEITTSHSV GNMPSP. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Inhibits the dipeptidyl peptidase activity of DPP4 . Cell surface proteoglycan that bears heparan sulfate. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. Reference: Isolation of a cDNA corresponding to a developmentally regulated transcript in rat intestine.Filmus J., Church J.G., Buick R.N.Mol. Cell. Biol. 8:4243-4249(1988). Function: Inhibits the dipeptidyl peptidase activity of DPP4 (By similarity). Cell surface proteoglycan that bears heparan sulfate. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.