Glycerol kinase (glpK), Escherichia coli, recombinant

Organism: Escherichia coli (strain K12). Source: E.coli. Expression Region: 2-502aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: TEKKYIVALD QGTTSSRAVV MDHDANIISV SQREFEQIYP KPGWVEHDPM EIWATQSSTL VEVLAKADIS SDQIAAIGIT NQRETTIVWE KETGKPIYNA IVWQCRRTAE ICEHLKRDGL EDYIRSNTGL VIDPYFSGTK VKWILDHVEG SRERARRGEL LFGTVDTWLI WKMTQGRVHV TDYTNASRTM LFNIHTLDWD DKMLEVLDIP REMLPEVRRS SEVYGQTNIG GKGGTRIPIS GIAGDQQAAL FGQLCVKEGM AKNTYGTGCF MLMNTGEKAV KSENGLLTTI ACGPTGEVNY ALEGAVFMAG ASIQWLRDEM KLINDAYDSE YFATKVQNTN GVYVVPAFTG LGAPYWDPYA RGAIFGLTRG VNANHIIRAT LESIAYQTRD VLEAMQADSG IRLHALRVDG GAVANNFLMQ FQSDILGTRV ERPEVREVTA LGAAYLAGLA VGFWQNLDEL QEKAVIEREF RPGIETTERN YRYAGWKKAV KRAMAWEEHD E. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate. It also catalyzes the phosphorylation of dihydroxyacetone, L-glyceraldehyde and D-glyceraldehyde. It uses only ATP. Reference: A single amino acid change in Escherichia coli glycerol kinase abolishes glucose control of glycerol utilization in vivo.Pettigrew D.W., Liu W.Z., Holmes C., Meadow N.D., Roseman S.J. Bacteriol. 178:2846-2852(1996). Function: Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate. It also catalyzes the phosphorylation of dihydroxyacetone, L-glyceraldehyde and D-glyceraldehyde. It uses only ATP.