Glycerol-3-phosphate dehydrogenase, mitochondrial (GPD2), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 43-727aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: ADCISEPVNR EPPSREAQLL TLQNTSEFDI LVIGGGATGS GCALDAVTRG LKTALVERDD FSSGTSSRST KLIHGGVRYL QKAIMKLDIE QYRMVKEALH ERANLLEIAP HLSAPLPIML PVYKWWQLPY YWVGIKLYDL VAGSNCLKSS YVLSKSRALE HFPMLQKDKL VGAIVYYDGQ HNDARMNLAI ALTAARYGAA TANYMEVVSL LKKTDPQTGK VRVSGARCKD VLTGQEFDVR AKCVINATGP FTDSVRKMDD KDAAAICQPS AGVHIVMPGY YSPESMGLLD PATSDGRVIF FLPWQKMTIA GTTDTPTDVT HHPIPSEEDI NFILNEVRNY LSCDVEVRRG DVLAAWSGIR PLVTDPKSAD TQSISRNHVV DISESGLITI AGGKWTTYRS MAEDTINAAV KTHNLKAGPS RTVGLFLQGG KDWSPTLYIR LVQDYGLESE VAQHLAATYG DKAFEVAKMA SVTGKRWPIV GVRLVSEFPY IEAEVKYGIK EYACTAVDMI SRRTRLAFLN VQAAEEALPR IVELMGRELN WDDYKKQEQL ETARKFLYYE MGYKSRSEQL TDRSEISLLP SDIDRYKKRF HKFDADQKGF ITIVDVQRVL ESINVQMDEN TLHEILNEVD LNKNGQVELN EFLQLMSAIQ KGRVSGSRLA ILMKTAEENL DRRVPIPVDR SCGGL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Calcium-responsive mitochondrial glycerol-3-phosphate dehydrogenase which seems to be a key component of the pancreatic beta-cell glucose-sensing device. Reference: "The sequence of a human mitochondrial glycerol-3-phosphate dehydrogenase-encoding cDNA." Lehn D.A., Brown L.J., Simonson G.D., Moran S.M., McDonald M.J. Gene 150:417-418(1994). Function: nan