Glycerate 2-kinase (gck), Pyrococcus horikoshii, recombinant

Organism: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3). Source: E.coli. Expression Region: 1-440aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MIAMDIREIG LRLVGEAIKA ADPYRAVLNA VKVSDDKIIV QGKEFEIKGK VYVIALGKAA CEMARAIEDI LDVEDGVAVT KYGYGKELKR IKVIEAGHPI PDEKSILGAK EALSILNRAR ENDIVFILIS GGGSALFELP EEGISLEDLK LTTDLLLKSG AKIHEINTVR KHISKVKGGK LAKMIKGTGI VLIISDVVGD NLEAIASGPT VKDPTTFEDA KRILELYDIW EKVPESVRLH IERGLRGEVE ETLKEDLPNV HNFLIASNSI SCEAIAREAQ RLGFKAYIMT TTLEGEAKDA GLFIGSIVQE IAERGRPFEP PVVLVFGGET TVTIEGKGGK GGPNQEIALS ATRKISDLEA LIVAFDTDGT DGPTDAAGGI VDGTTYKKLR EKGIDVEKVL KEHNSYEALK KVGGLLFTGP TGTNVNSIVI AIVTSKRGRT. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the ATP-dependent phosphorylation of D-glycerate to 2-phosphoglycerate. It can also utilize GTP, CTP, UTP, ADP or pyrophosphate as phosphate donor. Reference: "Crystal structure of the PH0495 protein from Pyrococccus horikoshii OT3." Mizutani H., Kunishima N. Submitted (MAY-2005). Function: nan