Glutamine synthetase (GLUL), Cricetulus griseus, recombinant

Organism: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Source: E.coli. Expression Region: 2-373aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: ATSASSHLNK GIKQMYMSLP QGEKVQAMYI WVDGTGEGLR CKTRTLDCEP KCVEELPEWN FDGSSTFQSE SSNSDMYLSP VAMFRDPFRK EPNKLVFCEV FKYNQKPAET NLRHTCKRIM DMVSNQHPWF GMEQEYTLLG TDGHPFGWPS DGFPGPQGLY YCGVGADKAY RRDIMEAHYR ACLYAGVKIT GTYAEVKHAQ WEFQIGPCEG IRMGDHLWVA RFILHRVCKD FGVIATFDSK PIPGNWNGAG CHTNFSTKTM REENGLKHIK EAIEKLSKRH RYHIRAYDPK GGLDNARRLT GFHKTSNIND FSAGVADRSA SIRIPRTVGQ EKKGYFEARC PSANCDPFAV TEAIVRTCLL NETGDQPFQY KN. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine. Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia. Essential for proliferation of fetal skin fibroblasts. Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation. May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ. Plays a role in ribosomal 40S subunit biogenesis. Reference: "The cloning and nucleotide sequence of cDNA for an amplified glutamine synthetase gene from the Chinese hamster."Hayward B.E., Hussain A., Wilson R.H., Lyons A., Woodcock V., McIntosh B., Harris T.J.R.Nucleic Acids Res. 14:999-1008(1986). Function: nan