Genome polyprotein, partial, Yellow fever virus, recombinant

Organism: Yellow fever virus (strain 17D vaccine) (YFV). Source: E.coli. Expression Region: 286-730aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged. Target Protein Sequence: AHCIGITDRD FIEGVHGGTW VSATLEQDKC VTVMAPDKPS LDISLETVAI DRPAEVRKVC YNAVLTHVKI NDKCPSTGEA HLAEENEGDN ACKRTYSDRG WGNGCGLFGK GSIVACAKFT CAKSMSLFEV DQTKIQYVIR AQLHVGAKQE NWNTDIKTLK FDALSGSQEV EFIGYGKATL ECQVQTAVDF GNSYIAEMET ESWIVDRQWA QDLTLPWQSG SGGVWREMHH LVEFEPPHAA TIRVLALGNQ EGSLKTALTG AMRVTKDTND NNLYKLHGGH VSCRVKLSAL TLKGTSYKIC TDKMFFVKNP TDTGHGTVVM QVKVSKGAPC RIPVIVADDL TAAINKGILV TVNPIASTND DEVLIEVNPP FGDSYIIVGR GDSRLTYQWH KEGSSIGKLF TQTMKGVERL AVMGDTAWDF SSAGGFFTSV GKGIHTVFGS AFQGL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Capsid protein C: Plays a role in virus budding by binding to the cell membrane and gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. During virus entry, may induce genome penetration into the host cytoplasm after hemifusion induced by the surface proteins. Can migrate to the cell nucleus where it modulates host functions. Reference: "Yellow fever 17D vaccine virus isolated from healthy vaccinees accumulates very few mutations." Xie H., Cass A.R., Barrett A.D.T. Virus Res. 55:93-99(1998). Function: Capsid protein C