Gamma-aminobutyric acid receptor subunit pi (GABRP), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 17-242aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: ERMCIQGSQF NVEVGRSDKL SLPGFENLTA GYNKFLRPNF GGEPVQIALT LDIASISSIS ESNMDYTATI YLRQRWMDQR LVFEGNKSFT LDARLVEFLW VPDTYIVESK KSFLHEVTVG NRLIRLFSNG TVLYALRITT TVACNMDLSK YPMDTQTCKL QLESWGYDGN DVEFTWLRGN DSVRGLEHLR LAQYTIERYF TLVTRSQQET GNYTRLVLQF ELRRNV. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. In the uterus, the function of the receptor appears to be related to tissue contractility. The binding of this pI subunit with other GABA(A) receptor subunits alters the sensitivity of recombinant receptors to modulatory agents such as pregnanolone. Reference: "GABA(A) receptor pi (GABRP) stimulates basal-like breast cancer cell migration through activation of extracellular-regulated kinase 1/2 (ERK1/2)." Sizemore G.M., Sizemore S.T., Seachrist D.D., Keri R.A. J Biol Chem 289:24102-24113(2014). Function: nan